DN32 iNKT cells and a cholangiocyte line (603B) were grown separately or in co-culture in the absence or presence of α –Galactosyleramide (α GalCer). After 24 h, supernatants was harvested and analyzed for secreted A) IL2, B) IL13, C) IL4, D) Interferon-γ – (IFNγ ), and E) IL10 by ELISA. Data show mean ± SEM from two experiments assayed in triplicate. *p<0.05 vs. all other groups; data analyzed using ANOVA.