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. Author manuscript; available in PMC: 2010 Oct 28.
Published in final edited form as: Eur J Immunol. 2009 Jul;39(7):1879–1892. doi: 10.1002/eji.200838890

Figure 7. Shh treatment up-regulates activation markers in DN32 iNKT cells.

Figure 7

DN32 iNKT cells were plated as described in Fig 2 and treated with vehicle or Shh (10 ng/ml) for 24 h. RNA was isolated and expression of the hedgehog target gene, gli1, and various iNKT cell activation markers were evaluated by qRT-PCR. A) gli1, B) CD69, C) CD154, D) CD178. Results are the mean (± SEM) of triplicate experiments. *p<0.05 vs. vehicle (Shh 0 ng/ml) treated cells, using Student’ s t-test.