DN32 iNKT cells were plated as described in Fig 2 and treated with vehicle or Shh (10 ng/ml) for 24 h. RNA was isolated and expression of the hedgehog target gene, gli1, and various iNKT cell activation markers were evaluated by qRT-PCR. A) gli1, B) CD69, C) CD154, D) CD178. Results are the mean (± SEM) of triplicate experiments. *p<0.05 vs. vehicle (Shh 0 ng/ml) treated cells, using Student’ s t-test.