Figure 1.

Sirt1 inhibition decreases ghrelin-induced c-fos expression in NPY/Agrp neurons. EX-527 (i.c.v., 1.5 nmol/mouse), a pharmacological inhibitor of Sirt1 (Napper et al., 2005; Pacholec et al., 2010), diminished the number of c-fos labeled NPY-GFP cells after ghrelin treatment (i.p.). a, Slices from the brain highlighting the ARC (in yellow) where the NPY-GFP and c-fos cells were counted (between bregma −1.50 mm to −1.70 mm). b, Histogram showing quantification of double-labeled NPY-GFP/c-fos cells in the ARC of mice injected with vehicle/EX-527 and vehicle/ghrelin. c–f, Representative pictures of double immunohistochemistry for GFP (DAB, brown) and c-fos (nickel DAB, black). White arrows indicate double-stained cells. n = 4–5 mice/group. *p < 0.05, **p < 0.01.