. Author manuscript; available in PMC: 2010 Oct 28.

Published in final edited form as: Gastroenterology. 1986 Jul;91(1):182–188. doi: 10.1016/0016-5085(86)90456-7

Table 1.

Specificity of Cytosolic and Nuclear Estrogen and Androgen Receptors

Substances	[³H]E₂ Binding remaining (%)		[³H]R1881 Binding remaining (%)
Substances	cER^a	nER^a	cAR^a	nAR^a
E₁	50	25	–	–
E₂	25	26	64	68
E₃	58	45	–	–
DES	40	30	98	97
R1881	100	94	19	27
Cortisone	100	93	75	–
Progesterone	100	–	95	–
Triamcinolone acetonide	–	–	48	100
Cyproterone acetate	–	–	56	74

cAR, cytosolic androgen receptor; cER, cytosolic estrogen receptor; DES, diethylstilbesterol; E_1, estrogen; E_2, estradiol; E₃, estriol; nAR, nuclear androgen receptor; nER. nuclear estrogen receptor.

Cytosol and nuclear fractions were prepared and incubated with ³H-steroid and either no competitor (100%) value or a 100-fold excess of the substances listed, as described in Materials and Methods. No triamcinolone acetonide was included in the assay except in its use as a potential competitor.