qPCR Analysis of Auxin-Related Genes.
Wild-type Arabidopsis (Columbia) seedlings were grown in LD for 7 DAG. The seedlings were then incubated for 0.5, 1, 2, 4, or 6 h in liquid medium with or without 5 μM t-Z. The root tips were harvested and genes related to auxin biosynthesis (A) or auxin signaling (B) were analyzed by qPCR. Error bars indicate se (n = 3).