Figure 10.

Effect of Filipin Treatment on the Distribution of GFP-RabA1d.

(A) GFP-RabA1d strictly accumulated in the apical dome of the growing root hair and completely colocalized with FM4-64 in control conditions. A 25-min treatment with 10 μg·mL⁻¹ filipin led to the formation of filipin-positive apical compartments that colocalized with the GFP-RabA1d signal. This is documented in a single median section view (B), in a whole z-projection view (B₁), and in a rotated image of a transversal section of the subapical zone (B₂). DIC image (top row in [A], [B], and [B₁]), single confocal planes (rows 2 and 3 in each panel), and confocal-DIC (last row in left panel in [A]), confocal GFP-FM4-64 (last row in right panel in [A]), and confocal filipin-GFP (last row in [B] to [B₂]) merged images. Bar = 5 μm.