Figure 8.

Motility of Endosomes Visualized by the Double FYVE-GFP Construct after Recovery from Filipin Treatment.

Root hairs treated with different concentrations of filipin for 25 min and washed out with fresh medium. Both cytoplasmic streaming and endosomal motility were completely recovered in root hairs pretreated with 1 μg·mL⁻¹ filipin (A) and 3 μg·mL⁻¹ filipin (B). The motility of endosomal compartments visualized by the double FYVE-GFP construct was also recovered in root hairs pretreated with 10 μg·mL⁻¹ filipin (C), but FYVE compartments in close contact with filipin-positive apical compartments (arrow in [C]) remained immobilized after recovery. Note the stable pattern of filipin staining after washing. DIC (top row) and single confocal planes (rows 2 to 4). Row 5 in each panel: representative image of GFP-DIC merged image from Supplemental Movies 13 (A), 14 (B), and 15 (C) online. Bar = 5 μm.