Figure 2.

Interaction and Function of COPT1, COPT5, and XA13 Proteins.

(A) COPT1 and COPT5 interact with themselves and each other in the plasma membrane of tobacco leaf cells as shown by yellow fluorescence signal. Bars = 20 μm.

(B) Functional complementation of S. cerevisiae ctr1Δctr3Δ mutant (MPY17) by coexpression of Xa13, COPT1, and COPT5. Complementation is indicated by growth on the media with 0 or 5 μM Cu. The p413, p414, and p416 are yeast expression vectors. Yeast ScCtr1 and empty vector (V) were used as positive and negative controls, respectively. Transformants were grown in SC-His-Trp-Ura medium to exponential phase and spotted onto SC-His-Trp-Ura, ethanol/glycerol (YPEG), and bathocuproine disulfonic acid (BCS) plates.