Table 1. Proteins identified by MALDI-TOF/TOF.
Band number | Accession number (gi)* | Protein name | Obs. Mr (kDa) | Theo. Mr (kDa) | Matched peptides | Score | Expect | Seq. Cov. (%)** |
---|---|---|---|---|---|---|---|---|
1 |
194595509 |
spectrin alpha chain, brain isoform 1 |
96.05 |
285.72 |
18 |
77 |
0.0041 |
8 |
2 |
23307793 |
Serum albumin precursor |
84.46 |
71.34 |
7 |
70 |
0.021 |
12 |
3 |
38154680 |
Lactoferrin |
74.21 |
79.81 |
19 |
358 |
3.4e-031 |
30 |
4 |
6013427 |
Serum albumin |
66.50 |
71.18 |
12 |
317 |
4.3e-027 |
16 |
5 |
70058 |
Ig alpha-2 chain C region |
62.45 |
37.35 |
3 |
66 |
0.052 |
14 |
6 |
25987833 |
Ig gamma-2 heavy chain constant region |
57.97 |
36.36 |
6 |
92 |
0.00012 |
20 |
7 |
4503571 |
alpha-enolase |
54.62 |
47.48 |
11 |
128 |
3.4e-008 |
25 |
8 |
14250401 |
actin, beta |
50.30 |
41.32 |
15 |
352 |
1.4e-030 |
46 |
9 |
13489087 |
leukocyte elastase inhibitor |
47.43 |
42.83 |
5 |
93 |
0.00012 |
16 |
10 |
4502101 |
annexin A1 |
37.48 |
38.92 |
4 |
74 |
0.0084 |
14 |
11 |
125145 |
Ig kappa chain C region |
29.42 |
11.77 |
2 |
126 |
5.4e-008 |
32 |
13 |
4504963 |
Lipocalin-1 precursor |
17.06 |
19.41 |
5 |
230 |
2.2e-018 |
26 |
14 |
4504963 |
Lipocalin-1 precursor |
16.40 |
19.41 |
1 |
49 |
0.0021 |
6 |
15 | 4557894 | Lysozyme precursor | 12.71 | 16.98 | 7 | 258 | 3.4e-021 | 35 |
The asterisk indicates the accession number (gi) was according to the NCBInr201005 database and the double asterisk indicates the amino acid sequence coverage for the identified proteins; Ig: Immunoglobulin.