Table 3. TaqMan® Copy Number Assays used for copy number analysis of SPARC.
| Assay ID | Reporter dye | Context sequence | Location on NCBI assembly |
|---|---|---|---|
| Hs02667978_cn |
FAM |
GTCTCAAAACCCCAGCTCAAAATAC |
151021358 |
| Hs06106867_cn |
FAM |
GTCAGAAGGTTGTTGTCCTCATCCC |
151027253 |
| Hs06124887_cn | FAM | CTTCCCAGAGGTGTGGATTAATGGT | 151046100 |
The three assays selected are for target sequences located in proximity to the 5′- and 3′-ends of and within the gene. Any assay(s) detected to have gain or loss of copy number(s) may indicate, at least partially, the copy number of the SPARC gene.