Scheme 1.
PEGylation of G3 and G5 PAMAM dendrimers with EGmNHS to provide PAMAM G3-%EGm and G5-%EGm. The amounts of EGmNHS are specified in Table 1. The degree of PEGylation and number of PEG chains (n) were calculated from the difference of Mws of the dendrimer before and after PEGylation, which were obtained from their MALDI-TOF mass spectra.