Table 2.
Minimum inhibitory weight (MICPA) and molar concentrations (MICPA/Mw) of the G3 and G5 PAMAM dendrimers against P. aeruginosa, and the corresponding concentrations of the peripheral amino groups (MICPA × NNH2/Mw)
| Compounda | Measured Mws from MALDIb/Da | PDIc | NNH2d | MICPA/μg mL−1 | MICPAMw−1/μM | MICPA × NNH2/Mw−1/μM |
|---|---|---|---|---|---|---|
| G3-84% EG7 | 17247 | 1.02 | 5 | >400 | — | — |
| G3-77% EG11 | 20464 | 1.02 | 7 | >400 | — | — |
| G3-52% EG7 | 13106 | 1.02 | 15 | >400 | — | — |
| G3-42% EG11 | 14424 | 1.02 | 18 | >400 | — | — |
| G3-10% EG7 | 8139 | 1.02 | 28 | >50 | — | — |
| G3-6% EG11 | 8282 | 1.02 | 29 | 25 | 3.0 | 87 |
| G3 | 6859 | 1.02 | 31 | 6.3 | 0.92 | 29 |
| G5-74% EG11 | 72841 | 1.01 | 29 | >400 | — | — |
| G5-65% EG7 | 55006 | 1.02 | 38 | >400 | — | — |
| G5-31% EG7 | 39713 | 1.03 | 76 | 50 | 1.3 | 99 |
| G5-16% EG11 | 36499 | 1.03 | 92 | 50 | 1.4 | 129 |
| G5-6% EG11 | 30599 | 1.03 | 103 | 25 | 0.82 | 84 |
| G5 | 26374 | 1.02 | 110 | 12.5 | 0.47 | 52 |
a
Percent PEGylations were calculated using the measured Mws from the MALDI spectra of the dendrimers.
b
Mws of the highest frequency fragment in the major peak from the MALDI spectra were obtained using the Voyager Data Explorer software.
c
PDIs were calculated using the Mn and Mw derived from the boxed regions selected in the MALDI-TOF mass spectra of the dendrimers.
d
NNH2 is the approximate number of the free amino groups.