. Author manuscript; available in PMC: 2010 Oct 28.

Published in final edited form as: Psychopharmacology (Berl). 2009 Mar 10;204(4):667–677. doi: 10.1007/s00213-009-1497-4

Table 3.

Mean ED₅₀ values, dose ratios and respective 95% CL for rate-decreasing effects of flunitrazepam, pregnanolone and ketamine determined before, during and after daily flunitrazepam treatment. During daily treatment with either one or two injections of 1 mg/kg of flunitrazepam, dose-effect curves were determined under two different conditions: when the treatment dose of flunitrazepam was last administered 1 hr before sessions and when the treatment dose flunitrazepam was last administered 25 hr before sessions with flunitrazepam vehicle administered before sessions.

	ED₅₀ (mg/kg)		Dose ratio^a

	Value	95% CL	Value	95% CL

Flunitrazepam
Before	1.60	0.96-2.23	--
During 1 × 1 mg/kg flunitrazepam
1 hr	7.59	2.81-12.4	6.88	-1.09-14.84
25 hr	5.09	1.82-8.37	4.31	-0.05-8.67
During 2 × 1 mg/kg flunitrazepam
1 hr	11.27^*	6.10-16.43	9.53	1.17-17.89
25 hr	3.84	-0.92-8.59	5.08	-4.17-14.32
After	3.55	1.56-5.53	2.89	0.69-5.08

Pregnanolone
Before	8.52	6.73-10.30	--
During 1 × 1 mg/kg flunitrazepam,
1 hr	2.10^*	1.04-3.16	0.27	0.09-0.45
25 hr	12.97^*	10.65-15.29	1.65	1.12-2.19
During 2 × 1 mg/kg flunitrazepam
1 hr	7.60	4.16-11.04	0.90	0.53-1.28
25 hr	13.73^*	10.84-16.62	1.70	1.18-2.22
After	9.75	7.83-11.66	1.22	0.82-1.63

Ketamine
Before	1.63	0.99-2.27	--
During 1 × 1 mg/kg flunitrazepam,
1 hr	2.78^*	2.08-3.48	2.12	1.01-3.23
25 hr	2.13	1.25-3.01	1.65	0.60-2.71
During 2 × 1 mg/kg flunitrazepam
1 hr	4.99^*	2.88-7.10	3.53	1.82-5.24
25 hr	1.52	0.75-2.30	1.03	0.45-1.61
After	1.32	0.84-1.79	0.89	0.56-1.22

Dose ratios are relative to ED₅₀ values determined before treatment.

indicates a significant difference from ED₅₀ determined before daily flunitrazepam treatment