Table 2.

Use of commonly used serine/threonine phosphatase inhibitors for in vitro assays

Inhibitor/Condition	Use
Microcystin-LR, Calyculin-A, Nodularin, Tautomycin	Potent inhibitors of both PP1 and PP2A (Swingle, Ni et al. 2007)
Okadaic Acid, Fostriecin	Potent inhibitors of PP2A; used to differentiate PP2A activity from PP1 (Swingle, Ni et al. 2007)
Ca2+ removal with excess EGTA	Prevents the activation of PP2B (calcineurin) by Calcium/Calmodulin
Mg2+ removal with excess EDTA	Inhibits PP2C activity
PP1 inhibitory proteins (pT34-DARPP32, pT35-Inhibitor-1, inhibitor-2)	Specific inhibition of PP1