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hVFF mRNA levels for MMP1, MMP2, TIMP3 and MMPs/TIMP3 ratio on polystyrene and in Carbylan-GSX after treated with TNF-α (0.1 to 100ng/ml) in serum-free medium (DMEM-0.1BSA). Y axis is target gene (mRNA concentration ng/μl) normalized by the housekeeping gene, β-Actin (ng/μl); x axis is TNF-α concentration. (A) MMP1 mRNA level; (B) MMP2 mRNA level; (C) TIMP3 mRNA level; (D) MMP1 to TIMP3 ratio; (E) MMP2 to TIMP3 ratio.
