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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Laryngoscope. 2010 Sep;120(9):1819–1825. doi: 10.1002/lary.21037

Table 1.

Primer Sequences and Products of Real-Time Polymerase chain reaction (RT-PCR)

Gene GenBank# Forward Primer Reverse Primer Size of PCR product
MMP1 NM_002421 5′-TGCAACTCTGACGTTGATCCCAGA-3′ 5′-ACTGCACATGTGTTCTTGAGCTGC-3′ 122bp
MMP2 NM_004530 5′-AGAAGGATGGCAAGTACGGCT-3′ 5′-AGTGGTGCAGCTGTCATAGGATGT-3′ 128bp
TIMP3 NM_000362 5′-TGATGCAGCACACACACAATTCCC-3′ 5′-AAGCTCTGTTATTCTGGCCTGGGT-3′ 102bp
Collagen I α-2 NM_000089 5′-AACAAATAAGCCATCACGCCTGCC-3′ 5′-TGAAACAGACTGGGCCAATGTCCA- 3′ 101bp
Collagen III α-1 NM_000090 5′-CCATTGCTGGGATTGGAGGTGAAA-3′ 5′-TTCAGGTCTCTGCAGTTTCTAGCGG- 3′ 187bp
Fibronectin NM_002026 5′-ACCTACGGATGACTCGTGCTTTGA-3′ 5′-CAAAGCCTAAGCACTGGCACAACA-3′ 116bp
TNFR NM_003842 5′-TGGAGTGCAATGGTGCAATCTTGG-3′ 5′-ACCTGTGGTCCCAGCTATTTGGAT-3′ 98bp
β-Actin NM_001101 5′-ACGTTGCTATCCAGGCTGTGCTAT-3′ 5′-CTCGGTGAGGATCTTCATGAGGTAGT-3′ 188bp