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. Author manuscript; available in PMC: 2011 Nov 15.
Published in final edited form as: J Comp Neurol. 2010 Nov 15;518(22):4591–4611. doi: 10.1002/cne.22480

Figure 14.

Figure 14

Quantitative in situ hybridization for HDC, MCH and orexin mRNA in the intermediate and caudal regions of the human hypothalamus. The suggested divisions are based in a combination of anatomical features and quantification of the signal intensity for each transcript. (A1-5) Subdivisions for HDC expression. (A1) Level of the caudal paraventricular nucleus. (A2) Level of the lateral tuberal nucleus. (A3) Premammillary level. (A4) Rostral mammillary level. (A5) Mammillary level. (B1-4) MCH distribution can be divided in three main levels: (B1) premammillary level, (B2), rostral mammillary level and (B3), posterior hypothalamic level, with the higher intensity of signal at the second level. (C1-4) Suggested divisions for orexin signal: (C1) Periventricular level. (C2) Rostral premammillary level. (C3) Caudal premammillary level. (C4) Rostral mammillary level. Asterisk denotes p<0.05 versus “region one” within the rostro-caudal extent of analysis for each probe.