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CypB knockdown results in the decreased PRL-induced cell motility. Cell motility was assayed using Boyden chamber migration assay. The inserts were coated with collagen I overnight. T47D cells were arrested in the FBS-free medium and placed in the inserts. Cells were cultured for 20 h, and the migrated cells were counted under a microscope. Statistical analysis was performed using two-way ANOVA. ***P<0.001.
