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. 2010 Oct 28;5(10):e13692. doi: 10.1371/journal.pone.0013692

Figure 1. A schematic representation of the fluorescence recovery assay.

Figure 1

Based on the high specificity of the fluorescent probe BODIPY 493/503 for lipid droplets, we designed a rapid fluorimetric method for the evaluation of lipid droplets inside yeast cells. With this method, BODIPY's fluorescence is quenched in solution and, when cells are added, fluorescence is recovered due to passive BODIPY dislocation from the medium to lipid droplets, from which the quencher is excluded.