Skip to main content
. 2010 Oct 28;5(10):e15400. doi: 10.1371/journal.pone.0015400

Figure 1. C. albicans Rac1 accumulates in the nucleus.

Figure 1

(A) Intracellular accumulation of GFP-Rac1 in the absence of cell agitation. DIC and fluorescence images of rac1Δ/rac1Δ PADH1GFPRAC1 (PY205) cells from shaking cultures, after 1 h without agitation, followed by 1 h of shaking. Bar, 5 µm. (B) Rac1 accumulates in the nucleus in the absence of cells agitation. Intracellular GFP-Rac1 co-localizes with 4′,6-diamidino-2-phenylindole (DAPI). (C) Time-course of Rac1 nuclear accumulation. Cells with nuclear fluorescent signal were counted after the indicated times in the absence of agitation. The average of four experiments is shown with approximately 50 cells counted at each time point in each experiment (bars indicate standard deviation). (D) Rac1 accumulates in the nucleus of serum-induced hyphal cells. Hyphal growth in PY205 cells was induced in rich (YEPD) liquid media containing 50% FCS, for the indicated times, in the presence or the absence of agitation at 37°C.