Table 2. Protective efficacies of AMA1-BBVs against challenge infectiona.
| Vaccine (Challenge parasite) | Route | Anti-AMA1 D3 antibody titerc mean±SE | Anti-MSP119 antibody titerc mean±SE | No. of protected mice/total no. (%) |
| EXP4 (P. yoelii) | ||||
| G1: Non-immunized | - | NDd | ND | 0/5 (0) |
| G2: AcNPV-PyAMA1-D123surf | i.m. | 20,900±8,700 | ND | 5/10 (50) |
| G3: AcNPV-PyAMA1-D123surf | i.n. | 7,200±2,470 | ND | 2/10 (20) |
| G4: AcNPV-PyAMA1-D3surf | i.m. | 26,100±6,350 | ND | 0/5 (0) |
| G5: AcNPV-PyAMA1-D3surf | i.n. | 12,300±2,450 | ND | 0/5 (0) |
| G6: AcNPV-PyMSP119surf | i.m. | ND | 70,800±11,210 | 2/5 (40) |
| G7b: AcNPV-PyAMA1-D123surf + AcNPV-PyMSP119surf | i.m. | 15,200±5,800 | 94,600±15,720 | 5/5 (100) |
| EXP5 (P. berghei) | ||||
| G1: Non-immunized | - | ND | ND | 0/5 (0) |
| G2: AcNPV-PbAMA1-D123surf | i.m. | 9,800±3,010 | ND | 0/5 (0) |
| G3: AcNPV-PbAMA1-D3surf | i.m. | 16,900±5,310 | ND | 0/5 (0) |
a
Groups of BALB/c mice were immunized with AMA1-BBVs three times and challenged either with P. yoelii or P. berghei following blood collection for ELISA.
b
The two BBVs (AcNPV-PyAMA1-D123surf and AcNPV-PyMSP119surf) were mixed (2.5×107 pfu each) and used for immunization.
c
Levels of PyAMA1D3 and PyMSP119- specific IgGs and PbAMA1D3 and PbMSP119- specific IgGs for EXP4 and 5, respectively, were measured by ELISA.
d
ND, not detectable level (<500).