Figure 2.

Transiently expressed ALMS1 deletion constructs appear to displace endogenous ALMS1 from the centrosome. U2OS cells were transfected with HA-tagged constructs ΔN-3175 or 2261–2602, representing two putative centrosome-targeting domains in ALMS1, and costained with antibodies to HA and either endogenous ALMS1 (A) or γ-tubulin (B). The epitope recognized by the ALMS1 antibody is not present in either deletion construct, allowing constructs to be distinguished from endogenous ALMS1. The mean intensity of ALMS1 and γ-tubulin immunofluorescence in cells expressing each construct, relative to that in untransfected (UT) cells, is shown; 11–20 cells were analyzed for each condition.