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. 2010 Nov 1;21(21):3617–3629. doi: 10.1091/mbc.E10-03-0246

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 5. — siRNA-mediated depletion of KIAA1731 leads to progressive loss of centrioles and spindle pole abnormalities. (A) Representative images of siRNA-treated cells stained with an antibody to acetylated tubulin (green) to label centrioles. The number of clearly visible centrioles is indicated. The chart shows the percentage of cells containing >2, 2, 1, and 0 centrioles after 48-, 72-, and 96-h siRNA treatment. Approximately 120 cells were counted for each experimental condition. (B) Examples of mitotic siRNA-treated cells costained with antibodies to acetylated tubulin and γ-tubulin. Size bars, 5 μm.