Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Nov 1;21(21):3722–3734. doi: 10.1091/mbc.E10-08-0693

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

PMC Copyright notice

Figure 10. — Authentic ScRlg1p interacts with both HAC1^u and HAC1ⁱ mRNAs. Logarithmically growing yeast cells with no (wt), KAP95-TAP (KAP95-TAP), or ScRLG1-protein A (ScRLG1-prot. A) gene integrated into the chromosome were treated with (+Tm) or without (−Tm) Tm for 30 min and then were analyzed by CLIP. After cross-linking with 1.0% formaldehyde for 30 min, extracts were prepared and subjected to IP with IgG-Sepharose. Immunoprecipitated HAC1 mRNAs (A) and ACT1 mRNA (B) were amplified by RT-PCR with primers represented as thick arrows in schematic drawings of the genes. T, total extract; FT, flow through; RNase IP, immunoprecipitate from the extract treated with RNase A; IP, immunoprecipitate. In A, recovery of HAC1 mRNAs (HAC1^u in the absence of Tm and HAC1ⁱ in the presence of Tm) in the immunoprecipitates was quantified by semiquantitative RT-PCR with a microchip electrophoresis system. The values were obtained from three independent IPs.