Figure 6.

FISH analysis of chromosomal regions that were identified by Illumina deep sequencing to be associated with nucleoli. FISH analysis was carried out on interphase HT1080 cells (I, II, and IV) or on a human lymphocyte metaphase chromosome spread (panel III). Probes detected either 4p telomere (green) and 4q telomere (red; I), 2p telomere (green) and 2q telomere (red; (II) or chr17q22 (green) and chr17q21 (red; III and IV). Nucleoli were labeled with an anti-nucleolin antibody and shown in blue (white arrowheads, I, II, and IV). Total DNA was stained with DAPI in the metaphase spread (gray, III). Nucleolar-associated loci are indicated with yellow arrows and nonassociated loci with white arrows. Scale bars, 10 μm.