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. 2010 Nov 1;21(21):3735–3748. doi: 10.1091/mbc.E10-06-0508

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 9. — Photoactivation of nucleolar-associated chromatin to compare its localization in parental and daughter cells following mitosis. Photoactivation of PA-GFP-H2B stably expressed in HeLa cells. ROIs were either nucleolar-associated (I and II) or separate from nucleoli in the nucleoplasm (III and IV). These ROIs were photoactivated at 406 nm (blue arrows), and representative midplane deconvolved images are shown from cells either before (I and III) or after (II and IV) mitosis. Both postmitotic images show daughter cells 4.5 h after the onset of telophase. PA-GFP-H2B is shown in green, and nucleoli are labeled with mCherry-B23 (red). White arrows mark photoactivated chromatin that relocalizes at nucleoli in daughter cells; yellow arrowheads point at chromatin that relocalizes at the nuclear periphery in daughter cells. Scale bars, 10 μm.