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Unwinding substrates utilized for NS3 assay. The strand containing the single-stranded overhang is defined as the loading strand and the complementary strand as the displaced strand. The substrates are labeled according to the strands of the duplex (D, DNA; R, RNA; P, PNA; S, phosphorothioate; M, morpholino). DD1 and DD2 differ only in the orientation of the duplex region, while DD3 and DD4 comprise totally different duplex regions. The base sequence of RR is identical to DD1.
