Table 1. Incorporation kinetics and relative fidelity of CVB3 RdRp variants.

Enzyme	kobs (s−1) correct nta	kobs (s−1) incorrect ntb	Relative Fidelityc
wild type	8.2±0.5	0.006±0.001	1367
A372V	7.5±0.5	0.003±0.001	2500
S299T	7.4±0.2	0.008±0.001	925

^a1 mM ATP, determined from stopped-flow fluorescence assay for AMP incorporation.

^b5 mM GTP, determined from ³²P-labeled primer-extension assay for GMP misincorporation.

^ck_{obs correct}/k_{obs incorrect}.