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. 2010 Oct 28;6(10):e1001167. doi: 10.1371/journal.ppat.1001167

Figure 2. Mature Gag and Env localize to the uropod.

Figure 2

Primary CD4+ T cells and P2 cells were infected with a VSV-G-pseudotyped HIV-1 encoding Gag-iYFP (green) (A and B) or Gag-YFP (green) (C). A) For detection of mature Gag, cells were fixed, permeabilized, and immunostained with anti-p17MA (red) as described in Materials and Methods and observed with an epifluorescence microscope. B) and C) For detection of Env on the cell surface, infected cells were incubated with anti-gp120 (IgG1 b12) and subsequently with AlexaFluor-594-conjugated anti-human IgG prior to fixation as described in Materials and Methods.