Table 1. Quantification of T Cell Polarization.

	Gag-Expressing Cells			Non-Gag-Expressing Cells
	Polarized (% of total)	Unpolarized (% of total)	Total	Polarized (% of total)	Unpolarized (% of total)	Total
Primary CD4+ T cells	253 (64.4%)	140 (35.6%)	393	530 (56.6%)	406 (43.4%)	936
P2 cells	176 (51.9%)	163 (48.1%)	339	97 (47.1%)	109 (52.9%)	206

Primary CD4⁺ T cells and P2 cells infected with VSV-G-pseudotyped HIV-1 encoding Gag-YFP were cultured for two days, fixed, immunostained for cellular proteins, and examined by fluorescence microscopy. Gag-YFP-positive and -negative cells were categorized based on cell morphology, and cells in each category were counted. Cells with circularity below 0.8 (see Materials and Methods) were categorized as polarized cells.