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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Fungal Genet Biol. 2010 Jun 30;48(1):49–61. doi: 10.1016/j.fgb.2010.06.012

Fig. 9.

Fig. 9

Overview of proteomic techniques for detecting the expression of thiotemplate enzymes in vivo. A) The PrISM workflow incorporates the “Ppant ejection assay” for the selective identification of thiotemplate enzymes. The workflow utilizes tandem mass spectrometric data of thiotemplate enzymes as a starting point for the cloning and analysis of thiotemplate pathways. B) OASIS utilizes thiotemplate-specific metabolic labeling with affinity tags to allow for enrichment and identification of thiotemplate pathways.