Skip to main content
. Author manuscript; available in PMC: 2011 Nov 3.
Published in final edited form as: J Am Chem Soc. 2010 Nov 3;132(43):15112–15115. doi: 10.1021/ja106508f

Figure 3.

Figure 3

(A) ppp polarized SFG amide I band of a 8-deletion mutant-Cyt-b5 in a dDMPC/dDMPC lipid bilayer as a function of temperature. The increase in the intensity of ppp polarized SFG amide I band indicates a reorientation of the protein. The intensity of ppp polarized SFG amide I band at 45° is lower compared to that of 40°, which can be attributed to the desorption of protein from the lipid bilayer surface. (B) Tilt angle of a 8-deletion mutant-Cyt-b5 as a function of temperature determined using SFG ppp/ssp signal strength ratio.