Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Schematic description of a RT-PCR assay for a target miRNA. Stem-loop primers, are first hybridized to the miRNA followed by reverse transcription. The resulting transcript is then quantitated using conventional real-time PCR, using a TaqMan probe. Figure adapted from reference 83.
