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Schematic diagram of the enzymatic ligation-based real-time PCR assay for measurement of mature miRNAs. In the presence of the target miRNA, two stem-loop probes, each of which is partially complementary to the target, brought into close proximity via hybridization with the miRNA. T4 ligase is then used to attach the probes together, forming an extended primer than is amenable to real-time PCR-based quantitation. Figure adapted from reference 85.
