. Author manuscript; available in PMC: 2011 Jun 15.

Published in final edited form as: J Immunol. 2010 May 14;184(12):7268–7280. doi: 10.4049/jimmunol.1000600

Table I.

Frequency of adult IL-17A- and IL-22-producing human Vγ2Vδ2 T cells¹

Donor	PBMC per ml blood	% Vδ2 among CD3 T cells	% IL-17A⁺ among Vδ2	% IL-22⁺ among Vδ2	Frequency IL-17A⁺ Vδ2 among CD3 T cells	IL-17A⁺ Vδ2 per ml blood	Frequency IL-22⁺ Vδ2 among CD3 T cells	IL-22⁺ Vδ2 per ml blood
1	1.70×10⁶	3.99	0.61	0.64	1:4,109	239	1:3,916	256
2	2.36×10⁶	1.81	1.96	1.60	1:2,819	283	1:3,456	142
3	1.45×10⁶	2.04	2.29	2.24	1:2,141	522	1:2,188	421
4	1.93×10⁶	3.58	0.44	0.93	1:6,348	147	1:3,004	309
5	3.33×10⁶	4.18	0.19	0.46	1:12,591	67	1:5,201	167
6	1.89×10⁶	6.88	0.26	0.62	1:5,590	246	1:2,344	454
7	3.57×10⁶	23.9	0.35	0.98	1:1,195	1213	1:427	3567
8	1.23×10⁶	3.68	3.00	2.10	1:906	728	1:1,294	580
9	2.25×10⁶	1.24	0.38	1.81	1:21,222	80	1:4,456	293
10	2.82×10⁶	1.23	1.23	0.59	1:6,610	367	1:13,780	203
Mean±SEM	2.25±2.46×10⁶	5.25±2.14	1.07±0.32	1.20±0.21	1:2,762±1:9,047	389±112	1:1,864±1:4,775	639±328

PBMC were harvested from normal adult donors, counted, and stimulated with 50 ng/ml PMA and 2 μg/ml Ionomycin for 4–6 h in the presence of GolgiSTOP (monensin). PBMC were then stained with Live/Dead Blue (Invitrogen) followed by APC-Cy7-conjugated anti-CD3, FITC conjugated anti-Vδ2 or FITC-conjugated anti-TCR γδ. The cells were then washed, fixed, and permeabilized using the BD Cytofix/Cytoperm Kit and then intracellularly stained with either PE-conjugated anti-IL-17A alone or PE-conjugated anti-IFN-γ or PE-conjugated anti-IL-22 combined with PerCP-Cy5.5-conjugated anti-IL-17A.