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. Author manuscript; available in PMC: 2011 Mar 1.

Published in final edited form as: Cell Signal. 2010 Mar;22(3):377–385. doi: 10.1016/j.cellsig.2009.10.007

DU-145 cells were pretreated with PP2 (20 μM, 1 h) and treated with TRAIL (100 ng/ml) for various times (0-4 h). After treatment, cells were harvested and western blot analysis was performed for detecting PARP, phosphorylated Akt and Akt (A). Morphological features were analyzed with a phase-contrast inverted microscope (B). Cell survival was determined by trypan blue exclusion assay (C). DMSO: 1% dimethyl sulfoxide treated sham control. T-1, T-2, and T-4 represent TRAIL treatment for 1 h, 2 h, and 4 h, respectively.

DU-145 cells were pretreated with PP2 (20 μM, 1 h) and treated with TRAIL (100 ng/ml) for various times (0-4 h). After treatment, cells were harvested and western blot analysis was performed for detecting PARP, phosphorylated Akt and Akt (A). Morphological features were analyzed with a phase-contrast inverted microscope (B). Cell survival was determined by trypan blue exclusion assay (C). DMSO: 1% dimethyl sulfoxide treated sham control. T-1, T-2, and T-4 represent TRAIL treatment for 1 h, 2 h, and 4 h, respectively.