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. 2010 Aug 17;103(7):1040–1047. doi: 10.1038/sj.bjc.6605847

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Copyright © 2010 Cancer Research UK

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TGF-β1 present in medium conditioned by mammary tumour cells is responsible for the upregulation of NOX4 expression in RMF-EG cells. Serum-deprived stromal RMF-EG cells were incubated for 24 h with medium conditioned by MCF-7 and MDA-231 cells in the presence or absence of 10 μM SB431542. NOX4 mRNA expression was evaluated through qPCR as described in the Materials and Methods. ^*P<0.05, ^**P<0.01.