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. 2010 Aug 17;103(7):975–986. doi: 10.1038/sj.bjc.6605859

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Copyright © 2010 Cancer Research UK

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Effect of COX-2 silencing mediated by InvColi-pS on CRC cell invasiveness. Invasion index was evaluated in (A) HT-29 and (B) HCA-7 cells, 48 h after InvColi strain infection. The invasion assay was performed using Boyden chambers and 8-μm polycarbonate membranes coated with Matrigel (40-fold dilution). Samples were tested in the absence (dark bars) and in the presence (light bars) of PMA 40 nM. Relative invasion index refers to HT-29 and HCA-7 cells infected with InvColi-pS⁻, not treated with PMA. Data represent mean±s.e.m. of three independent experiments. ^*P<0.01.