Figure 8.

InvColi-pS infection impairs proliferation, viability and invasiveness of HCT-116 cells. (A) Cell number, (B) cell viability and (C) invasiveness were evaluated in HCT-116 cells infected by InvColi-pS strains (MOI 1:100). Viability was evaluated using the Trypan Blue exclusion assay and quantified as ((number of unstained cells)/(number of total cells)) × 100. (⧫): cells not infected, NI; (▪): cells infected with InvColi-pS⁻; (▴): cells infected with InvColi-pS^H1; (•): cells infected with InvColi-pS^COX−2; (^*): cells infected with InvColi-pS^TBE. The invasion assay was performed using Boyden chambers and 8-μm polycarbonate membranes coated with Matrigel (40-fold dilution). Relative invasion index refers to HCT-116 cells not infected (NI). Data represent mean±s.e.m. of three independent experiments. ^*P<0.01.