Figure 2.

PCNA Immunocytochemistry (a–e) and TUNEL method (f–j) in the ventral prostate of 1 week control (a and f), 1 week diabetic (b and g), 1 week diabetic insulin-treated (c and h), 3 months control (d and i) and 3 months diabetic rats (e and j). Note that proliferation in prostate epithelium decreases significantly in older group (d) and also in diabetic groups, especially in short-term (b). The levels of apoptosis increased in prostate after diabetes (g and j), mainly in long-term rats (j). Insulin treatment completely reverses apoptotic levels but not cell proliferation in prostatic epithelium. Scale bar: 20 μm.