Figure 3.

Immunocytochemistry for androgen receptor AR (a–e) and p63 (f–j) in the ventral prostate of 1 week control (a and f), 1 week diabetic (b and g), 1 week diabetic insulin-treated (c and h), 3 months control (d and i) and 3 months diabetic rats (e and j). The frequency of AR significantly decreased because of ageing (d), but not because of diabetes (b and e), although a lower intensity of immunoreaction could be observed in short-term diabetic group (b). The number of p63-positive cells is significantly greater in diabetic rats (g and j) and insulin treatment reduced its expression levels close to those observed in control group (h). Scale Bar: 20 μm.