Force spectra collected by AFM in a buffer solution. Shown are randomly selected retraction traces for the following pairs (from top to bottom): Fn-coated tip on a clinical isolate of S. aureus (topmost curves), Fn-coated tip on a mutant strain of S. aureus that overexpresses FnBP on its outer surface, Fn-coated tip on a mutant strain of L. lactis that overexpresses FnBP on its outer surface, Fn-coated tip on a mutant strain of S. aureus that cannot express FnBP on its outer surface, BSA-coated tip on a mutant strain of S. aureus that overexpresses FnBP on its outer surface, and Fn-coated tip on a glass slide (bottom-most curves). See Buck et al. (74) for a complementary study. (Color figure online.)