Table 1.
Statistics of single Syb2 vesicle docking and fusion events recorded with model membranes of different lipid compositions with up to 5 mol % PS
| Second “cytoplasmic” leaflet lipid composition of supported membrane∗ | Syb2 vesicle lipid composition | Total number of supported bilayers | Total number of docking events | Total number of fusion events |
|---|---|---|---|---|
| POPC/Chol | POPC/Chol | 30 (28)† | 3205 | 1363 |
| POPC/DOPS/Chol | POPC/DOPS/Chol (75:5:20) | 18 (11) | 483 | 161 |
| POPC/Chol | 3 (2) | 112 | 28 | |
| POPC/DOPS/DOPE/Chol | POPC/DOPS/DOPE/Chol (70:5:5:20) | 18 (16) | 3255 | 1135 |
| POPC /Chol | 16 (15) | 2205 | 674 | |
| POPC/DOPS/DOPE/Chol | POPC/DOPS/DOPE/Chol (65:5:10:20) | 18 (15) | 2824 | 781 |
| POPC/Chol | 16 (13) | 2595 | 754 | |
| POPC/DOPS/DOPE/Chol | POPC/DOPS/DOPE/Chol (55:5:20:20) | 18 (9) | 1661 | 223 |
| POPC/Chol | 17 (15) | 2823 | 328 | |
| POPC/DOPS/DOPE/Chol | POPC/DOPS/DOPE/Chol (45:5:30:20) | 18 (13) | 1218 | 77 |
| POPC/Chol | 16 (16) | 3142 | 416 |
∗
First “extracellular” leaflet of all supported membranes consisted of POPC/Chol (4:1).
†
Numbers in parentheses represents total number of bilayers in which fusion and docking of Syb2 vesicles was observed.