Table 2.
Analysis of single vesicle fusion kinetics in model membranes of different lipid compositions
| Second “cytoplasmic” leaflet lipid composition of supported membrane∗ | Syb2 vesicle lipid composition | Number of particles per fusion site (m) | Fast fraction activation rate (s−1) | Fast fraction (%) | Slow fraction activation rate (s−1) |
|---|---|---|---|---|---|
| POPC/Chol | POPC/Chol | 8 ± 2 | 136 ± 15 | 87 ± 2 | 10.3 ± 1.2 |
| POPC/DOPS/DOPE/Chol | POPC/DOPS/DOPE/Chol (70:5:5:20) | 8 ± 2 | 112 ± 16 | 68 ± 4 | 9.2 ± 1.1 |
| POPC/Chol | 5 ± 1 | 89 ± 17 | 70 ± 6 | 5.2 ± 1.4 | |
| POPC/DOPS/DOPE/Chol | POPC/DOPS/DOPE/Chol (65:5:10:20) | 3 ± 1 | 86 ± 25 | 48 ± 9 | 11.2 ± 1.7 |
| POPC/Chol | 7 ± 2 | 138 ± 22 | 67 ± 4 | 12.7 ± 1.4 | |
| POPC/DOPS/DOPE/Chol | POPC/DOPS/DOPE/Chol (55:5:20:20) | N/A | |||
| POPC/Chol | N/A | ||||
| POPC/DOPS/DOPE/Chol | POPC/DOPS/DOPE/Chol (45:5:30:20) | N/A | |||
| POPC/Chol | 3 ± 2 | 74 ± 49 | 41 ± 23 | 10 ± 3 | |
∗
First “extracellular” leaflet of all supported membranes consisted of POPC/Chol (4:1).