Table 1.

Effect of CyA and FK 506^* on Hepatocyte DNA Synthesis.

Group No.	Addition to the Incubation Medium (MEM + Insulin)	[3H]-Thymidine incorporation (cpm × 10−3/mg DNA)
		% Serum	5	10	20	30	40
1	NRS		110 ± 15	88 ± 9	71 ± 14	53 ± 5	63 ± 7
2	NRS + CyA		92 ± 10	82 ± 10	56 ± 8	57 ± 7	38 ± 7
3	NRS + FK 506		86 ± 11	83 ± 4	59 ± 9	52 ± 6	36 ± 7
4	Vehicle-treated rat serum		130 ± 18	124 ± 10	106 ± 6	82 ± 10	68 ± 8
5	CyA-treated rat serum		140 ± 7	167 ± 11*	196 ± 12*	170 ± 11*	114 ± 10*
6	FK 506.treated rat serum		154 ± 16	306 ± 28†	286 ± 26†	244 ± 17†	225 ± 15†

Note: Culture conditions are the same as described in Fig 3, the only difference being that rat serum was used as mitogen instead of epidermal growth factor. In groups 2 and 3, the concentration in the incubation medium of FK 506 and CyA vehicles was <0.2 mg/ml; this concentration did not affect hepatocyte proliferation (data not Shown). The animals used in groups 4–6 to provide serum were treated as described in Fig 2, the difference being that on the fourth day, they only received the last injection and the blood was drawn 2 hours later. The values reported are the means of five experiments ± SD.

Abbreviations: MEM, minimum essential medium: NRS, normal rat serum.

^*Significantly different from group no. 4 (P < 0.05).

^†Significantly different from groups no. 4 (P < 0.02) and 5 (P < 0.01).