Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Aug 26;285(45):34330–34336. doi: 10.1074/jbc.M110.166314

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 2. — Identification of an altered molecule in ΔkdhA mutant outer membrane. A and B, outer membrane fractions from wild-type LVS and ΔkdhA mutant bacteria were resolved by SDS-PAGE and analyzed by zinc stain (A) and by immunoblot using anti-LVS serum (B). C, identification of band d as lipid A plus core plus the first repeating unit of O-polysaccharide. LVS outer membranes were resolved by SDS-PAGE and immunoblotted with mAb 2033 monoclonal antibody. D, identification of band c as lipid A plus core oligosaccharide by negative-ion MALDI-TOF MS. The band c molecule was micropurified from wild-type outer membranes as described under “Experimental Procedures.” The purity of the band c molecule was monitored by SDS-PAGE followed by zinc stain. Band b was identified as lipid A using the same procedure (supplemental Fig. 2).