FIGURE 3.

HPAEC analysis of core oligosaccharide from wild-type and ΔkdhA mutant bacteria. A, side-chain Kdo detection after mild hydrolysis of LPS from wild-type and ΔkdhA mutant. Kdo peak was determined by co-chromatography with a Kdo standard. B, galactosamine, glucose, and mannose detection after TFA hydrolysis of LPS from wild-type and ΔkdhA mutant. C, molar ratio of galactosamine, glucose, mannose, and side-chain Kdo in LPS from wild-type and ΔkdhA mutant. D, structure of core oligosaccharide synthesized by F. tularensis LVS strain (16) and proposed structure synthesized by LVS ΔkdhA mutant.