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Enzyme activity and gene and protein expression of Hint1. A, relative Hint1 mRNA expression levels in clone A, Huh7, CEM, and primary human hepatocytes from two different donors determined by RT-PCR. B, Western blot showing expression of Hint1 protein in extracts from clone A, Huh7, CEM, and primary human hepatocytes from two different donors. The same amount of protein was loaded on the gel for the Western blot analysis.
