TABLE 1.

Proteins interacting with the Lsm protein

Co-immunoprecipitation with the FLAG-Lsm fusion protein revealed several proteins associated specifically with the Lsm protein (proteins co-purified with the control were subtracted). Proteins are grouped into functional classes. The number of obtained MS/MS spectra is shown. Only proteins that were present in all three FLAG-Lsm purifications with at least four MS/MS spectra in each of the three independent isolations are listed. The complete list of identified proteins is shown in supplemental Table 3. In addition, supplemental Table 3 lists the accession numbers and the number of MS/MS spectra for all three replicas.

	Protein	Number of MS/MS spectra
Translation
1	Translation elongation factor aEF-2	47
2	Translation elongation factor aEF-1 α subunit	29
3	Ribosomal protein S3	10
4	Threonyl-tRNA synthetase	9
5	Ribosomal protein S3a.eR	8
6	Valyl-tRNA synthetase	7
Stress-related
7	Heat shock protein Cct2	32
8	Heat shock protein Cct1	30
9	CBS domain pair, putative	16
10	Thermosome subunit 3	10
11	UspA domain protein	7
Nucleic acid metabolism
12	DNA-directed RNA polymerase subunit A	15
13	mRNA 3-end processing factor homolog	13
14	Sugar-specific transcriptional regulator TrmB	6
15	Putative nuclease	6
16	DNA-directed RNA polymerase subunit B	5
17	Replication factor C small subunit	4
Cell cycle
18	Cell division control protein 48	15
19	Cell division control protein 48	12
20	SMC-like protein Sph2	10
Diverse functions
21	Betaine aldehyde dehydrogenase	16
22	Chlorite dismutase family protein	12
23	Pyruvate-ferredoxin oxidoreductase α subunit	10
24	Predicted hydrolase	10
25	Aconitate hydratase, putative	10
26	Coiled-coil protein	9
27	Fumarate hydratase class II	9
28	Proteasome subunit α1	8
29	Putative orotatephosphoribosyl transferase	8
30	Phosphopyruvate hydratase	7
31	Aspartate carbamoyltransferase	7
32	Short chain family oxidoreductase	7
33	Inosine-5-monophosphate dehydrogenase	6