FIGURE 2.

Overoxidation of purified plant and cyanobacterial 2-Cys Prx in vitro. Purified recombinant 2-Cys Prx (25 μg of protein) from rice (Os), Anabaena (Ana), and Synechocystis (Syn) were incubated with 10 mm DTT and 5 mm hydrogen peroxide. At the indicated times, the reaction was stopped by protein precipitation with 5% trichloroacetic acid and washed twice with ice-cold acetone. Samples (0.3 μg of protein of the rice enzyme and 5 μg of Anabaena and Synechocystis enzymes) were then subjected to SDS-PAGE under reducing conditions. Western blot analysis was performed using antibodies against overoxidized 2-Cys Prx.